|
KMID : 0613820120220121712
|
|
Journal of Life Science
2012 Volume.22 No. 12 p.1712 ~ p.1717
|
|
|
Antioxidative Effect of S-allylmercaptocysteine Derived from Aged Garlic on Oxidation of Human Low Density Lipoprotein
|
|
Yang Seung-Taek
|
|
|
Abstract
|
|
|
|
Oxidation of low density lipoprotein (LDL) plays an important role in the development and progression of atherosclerotic disease. In this study, human LDL was isolated and oxidized using CuSO₄ in the presence or absence of S-allylmercaptocysteine. Oxidative modification of the LDL fraction was monitored by both the appearance of thiobarbituric acid substances (TBARS), an increase in electrophoretic mobility, and conjugated diene formation. The addition of S-allylmercaptocysteine reduced lipid peroxide formation, indicating it to be an effective antioxidant. The inhibition of LDL oxidation by 5~20μg/ml S-allylmercaptocysteine occurred in a dose-dependent manner, as assessed by the TBARS assay. S-allylmercaptocysteine at 20μg/ml almost completely inhibited the Cu²+ induced increases in electrophoretic mobility of LDL and almost completely inhibited conjugated diene formation. A more potent antioxidative activity was observed for S-allylmercaptocysteine than for either Vitamin C or dℓ-α-tocopherol. Thus, S-allylmercaptocysteine aid in preventing the development and progression of atherosclerotic disease.
|
|
|
KEYWORD
|
|
|
Low density lipoprotein, aged garlic, S-allylmercaptocysteine, antioxidative activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|